Useful Links & Tools
- MSDS
- Certificate of Analysis
- EX-CELL Serum-Free Media for CHO Cells - SAFC Biosciences Technical Bulletin (38 KB )
- Removing Surfactants from Serum-Free Suspension Media - SAFC Biosciences Technical Bulletin (55 KB )
- Preparing and Filtering Serum-Free Media - SAFC Biosciences Technical Bulletin (32 KB )
- 24326C Product Information (48 KB )
24326C
Sigma
EX-CELL® 302 Serum-Free Medium for CHO Cells
dry powder, without L -glutamine, without phenol red, without sodium bicarbonate
Description
| Application | EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems. |
| Caution | Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. |
| Legal Information | p3xFLAG is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| EX-CELL is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| description | for research or for further manufacturing use |
| sterility | sterile-filtered |
| form | dry powder |
| storage condition | protect from light |
| pH | 7.1-7.7 |
| suitability | suitable for cell culture |
| storage temp. | 2-8°C |
